Difference between revisions of "Part:BBa K1696003"
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Fig.1 Design of the part.
 
Fig.1 Design of the part.
  
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But we found the yield was no different between BL21(blank) and BL21+ldhE(experientmental).
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But we found the yield was no different between BL21(blank) and BL21+ldhE(experientmental). Shown in Fig2.
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<img src=" https://static.igem.org/mediawiki/parts/2/2a/LdhE-2.png" width="500px"/>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 13:26, 16 September 2015

L-lactate producing part1

L-Lactic acid, one of the most important chiral molecules and organic acids, is produced via pyruvate from carbohydrates in diverse microorganisms catalyzed by an NAD+-dependent L-lactate dehydrogenase (ldhA).[1] Similar metabolic pathway has been found in a variety of organisms, to distinguish the differences, we renamed the heterogenous L-lactate dehydrogenase ldhE while homogenous one keeps original.

We choose a strong promoter+ strong RBS to construct L-Lactate part. The NAD+-dependent L-lactate dehydrogenase gene is from Lactobacillus casei.

Fig.1 Design of the part.

But we found the yield was no different between BL21(blank) and BL21+ldhE(experientmental). Shown in Fig2.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 36
    Illegal BsaI.rc site found at 1058