Difference between revisions of "Part:BBa K1632000:Design"
JunKawamura (Talk | contribs) (→Materials and Methods) |
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sequence confirmed | sequence confirmed | ||
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===Materials and Methods=== | ===Materials and Methods=== | ||
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Gene synthesis by eurofins | Gene synthesis by eurofins | ||
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===References=== | ===References=== | ||
Revision as of 05:07, 17 September 2015
fim switch[deault ON](Tokyo_Tech/J23119)
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 345
Illegal NheI site found at 368 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 374
Illegal BamHI site found at 333 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
sequence confirmed
Materials and Methods
Please click here. (fim switch[default ON](Tokyo_Tech/J23119)_rbs_GFP Experiment Page)
Source
Gene synthesis by eurofins
References
Ian C. Blomfield et al. (1997) Integration host factor stimulates both FimB- andFimE-mediated site-specific DNA inversion that controlsphase variation of type 1 fimbriae expression in Escherichia coli. Molecular Microbiology 23(4), 705–717
John M. Abraham et al. (1985) An invertible element of DNA controls phase variation of type 1 fimbriae of Escherichia coli. Proc Natl Acad Sci U S A 82(17):5724-7
Matthew P. McCusker et al. (2008) DNA sequence heterogeneity in Fim tyrosine-integrase recombinase-binding elements and functional motif asymmetries determine the directionality of the fim genetic switch in Escherichia coli K-12. Molecular Microbiology 67(1): 171–187