Difference between revisions of "Part:BBa K1767004"
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We built this BioBrick to test the degradation of AHL by AiiA and how AiiA affects the system. | We built this BioBrick to test the degradation of AHL by AiiA and how AiiA affects the system. | ||
| − | You can use this BioBrick to produce TetR for a longer period compared to BioBrick BBa_K176003 as it lack the AiiA, which is an enzyme that degrades AHL hence AHL degrades slower. Hence the gene that will be cloned downstream of the TetR promoter will be expressed later in time. | + | You can use this BioBrick to produce TetR for a longer period compared to BioBrick BBa_K176003 as it lack the AiiA, which is an enzyme that degrades AHL hence AHL degrades slower. Hence the gene that will be cloned downstream of the TetR promoter will be expressed later in time. </p> |
| − | The scheme below explains how this BioBrick works : | + | <p>The scheme below explains how this BioBrick works :</p> |
| + | [[File: Technion_HS_Israel_K1767004_1.jpg|850px|center]] | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 11:17, 16 September 2015
P(Lac)IQ RBS LuxR ter ter luxpR RBS tetR ter ter
We designed and built a kill switch mechanism that based on the activation of a lethal gene downstream of an inducible promoter that is activated over time.
The presented part is the first and the second module of our designed genetic circuit but without AiiA which consist of a constitutive promoter (I14032) driving expression of LuxR followed by a Lux promoter (R0062) , driving expression of TetR.
We built this BioBrick to test the degradation of AHL by AiiA and how AiiA affects the system.
You can use this BioBrick to produce TetR for a longer period compared to BioBrick BBa_K176003 as it lack the AiiA, which is an enzyme that degrades AHL hence AHL degrades slower. Hence the gene that will be cloned downstream of the TetR promoter will be expressed later in time. </p>
The scheme below explains how this BioBrick works :
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 987