Difference between revisions of "Part:BBa K1679007"
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This is a device containing a promoter (BBa_J23101),a RBS (BBa_K115001) and a RFP coding sequence (BBa_E0010) on the pSB1C3, which can be easily transformed into E.coli.In theory,When temperature rise above 42°C,It can produce RFP.However,according to our experimental,It can't appear red. | This is a device containing a promoter (BBa_J23101),a RBS (BBa_K115001) and a RFP coding sequence (BBa_E0010) on the pSB1C3, which can be easily transformed into E.coli.In theory,When temperature rise above 42°C,It can produce RFP.However,according to our experimental,It can't appear red. | ||
| − | + | <span class='h3bb'>Sequence and Features</span> | |
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<partinfo>BBa_K1679007 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1679007 SequenceAndFeatures</partinfo> | ||
Revision as of 09:42, 16 September 2015
promoter+RNA thermometer+RFP
This is a device containing a promoter (BBa_J23101),a RBS (BBa_K115001) and a RFP coding sequence (BBa_E0010) on the pSB1C3, which can be easily transformed into E.coli.In theory,When temperature rise above 42°C,It can produce RFP.However,according to our experimental,It can't appear red.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 700
Illegal AgeI site found at 812 - 1000COMPATIBLE WITH RFC[1000]