Difference between revisions of "Part:BBa K1230000:Experience"
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===Applications of BBa_K1230000=== | ===Applications of BBa_K1230000=== | ||
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+ | {|width='80%' style='border:1px solid gray' | ||
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+ | <partinfo>BBa_K190019 AddReview </partinfo> | ||
+ | <I>iGEM Nagahama_2015</I> | ||
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+ | We improved the characterization of a previously existing BioBrick Part [https://parts.igem.org/Part:BBa_K1230000 BBa_K1230000] and submitted this improved BioBrick ''marA'' device as [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1653006 BBa_K1653006] to iGEM Registry. | ||
+ | In exsisting part's information of ''marA'', it gives ''E. coli'' resistance against kanamycin only. In this year, we confilmed that overepressing of ''marA'' gives ''E. coli'' resistance against geraniol as one of the terpene and decrease its intracellular concentration. This information is very beneficial for other iGEMers to production of organic substance that have toxicity using bacteria. | ||
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+ | '''The activator of AcrAB-TolC multidrug efflux pump exports some terpenes''' | ||
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+ | <br>[[File:MarA dev.png]][https://parts.igem.org/wiki/index.php?title=Part:BBa_K1653020 BBa_K1653020] | ||
+ | [[File:GC conc fig.png |650px|thumb|center|Fig. 1: Intracellular geraniol concentrations of ''E. coli'' JM109 (WT) and its overexpressing of ''marA'' strain, ''E. coli'' JM109 (''marA''). | ||
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+ | In this figure, intracellular content of geraniol was less in the strain ''E. coli'' JM109 (''marA'') than the strain ''E. coli'' JM109 (WT). The concentrations of intracellular geraniol from ''E. coli'' JM109 (''marA'') was 42.9 μg/ml, which was 40% lower than that from of ''E. coli'' JM109 (WT), 72.2 μg/ml. This figure is suggesting that internalized geraniol could be more efficiently exported through AcrAB-TolC efflux pump following the presumed activation of this gene by introducing the activator ''marA'' gene.]] | ||
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+ | <!-- DON'T DELETE --><partinfo>BBa_K190019 EndReviews</partinfo> | ||
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We improved the characterization of a previously existing BioBrick Part [https://parts.igem.org/Part:BBa_K1230000 BBa_K1230000] and submitted this improved BioBrick ''marA'' device as [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1653006 BBa_K1653006] to iGEM Registry. | We improved the characterization of a previously existing BioBrick Part [https://parts.igem.org/Part:BBa_K1230000 BBa_K1230000] and submitted this improved BioBrick ''marA'' device as [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1653006 BBa_K1653006] to iGEM Registry. | ||
In exsisting part's information of ''marA'', it gives ''E. coli'' resistance against kanamycin only. In this year, we confilmed that overepressing of ''marA'' gives ''E. coli'' resistance against geraniol as one of the terpene and decrease its intracellular concentration. This information is very beneficial for other iGEMers to production of organic substance that have toxicity using bacteria. | In exsisting part's information of ''marA'', it gives ''E. coli'' resistance against kanamycin only. In this year, we confilmed that overepressing of ''marA'' gives ''E. coli'' resistance against geraniol as one of the terpene and decrease its intracellular concentration. This information is very beneficial for other iGEMers to production of organic substance that have toxicity using bacteria. |
Revision as of 08:02, 17 September 2015
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1230000
Review number not understood iGEM Nagahama_2015 |
We improved the characterization of a previously existing BioBrick Part BBa_K1230000 and submitted this improved BioBrick marA device as BBa_K1653006 to iGEM Registry.
In exsisting part's information of marA, it gives E. coli resistance against kanamycin only. In this year, we confilmed that overepressing of marA gives E. coli resistance against geraniol as one of the terpene and decrease its intracellular concentration. This information is very beneficial for other iGEMers to production of organic substance that have toxicity using bacteria.
|
UNIQ2b640fa6dce4c7bc-partinfo-00000001-QINU
We improved the characterization of a previously existing BioBrick Part BBa_K1230000 and submitted this improved BioBrick marA device as BBa_K1653006 to iGEM Registry.
In exsisting part's information of marA, it gives E. coli resistance against kanamycin only. In this year, we confilmed that overepressing of marA gives E. coli resistance against geraniol as one of the terpene and decrease its intracellular concentration. This information is very beneficial for other iGEMers to production of organic substance that have toxicity using bacteria.
The activator of AcrAB-TolC multidrug efflux pump exports some terpenes
Resistance
User Reviews
UNIQ2b640fa6dce4c7bc-partinfo-00000002-QINU UNIQ2b640fa6dce4c7bc-partinfo-00000003-QINU