Difference between revisions of "Part:BBa K1653020"
Yoshiharu994 (Talk | contribs) |
Yoshiharu994 (Talk | contribs) (→Resistance) |
||
Line 16: | Line 16: | ||
]] | ]] | ||
− | [[File:MarA plate assay nishikawa last.png|650px|thumb|center|Fig. | + | [[File:MarA plate assay nishikawa last.png|650px|thumb|center|Fig. 3: Comparison of colony numbers after addition of geraniol solution. Time interval for treatment was set every 1 hour from 1 hour to 4 hours. A: ''E. coli'' JM109 (WT) + hexane; B: ''E. coli'' JM109 (''marA'') + hexane; C: ''E. coli'' JM109 (WT) + geraniol; D: ''E. coli'' JM109 (''marA'') + geraniol. |
− | As shown in Figs. 2 A and B, treatment with hexane of ''E. coli'' JM109 (WT) and of ''E. coli'' JM109 (''marA'') showed similar colony numbers during these treatment intervals to those of time zero. This result suggests that hexane at this concentration and duration of time for 4hours did not affect both cell growth. In contrast, treatment with geraniol of ''E. coli'' JM109 (WT) and of ''E. coli'' JM109 (''marA'') showed toxicities to both strains (Figs. | + | As shown in Figs. 2 A and B, treatment with hexane of ''E. coli'' JM109 (WT) and of ''E. coli'' JM109 (''marA'') showed similar colony numbers during these treatment intervals to those of time zero. This result suggests that hexane at this concentration and duration of time for 4hours did not affect both cell growth. In contrast, treatment with geraniol of ''E. coli'' JM109 (WT) and of ''E. coli'' JM109 (''marA'') showed toxicities to both strains (Figs. 3 B, C and D). If we watch the colony numbers carefully, ''E. coli'' JM109 (''marA'') had more than ''E. coli'' JM109 (WT) during these treatment intervals ((Figs. XX C and D). These results demonstrate that toxicity of the geraniol was less to the strain ''E. coli'' JM109 (''marA'') than the strain ''E. coli'' JM109 (WT). |
]] | ]] | ||
− | [[File:MarA shinsei yamamoto made.png|500px|thumb|center|700px|Fig | + | [[File:MarA shinsei yamamoto made.png|500px|thumb|center|700px|Fig 4. Colony numbers of Fig2 ]] |
Revision as of 05:25, 16 September 2015
marA.dev This device consists of R0011+B0034+K1653006+B0015. The following graphs show that overexpressing marA gives resistance against geraniol and decrease intracelluler geraniol concentration
Export
Resistance
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 229
- 1000COMPATIBLE WITH RFC[1000]