Difference between revisions of "Part:BBa M36247"
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== Improvement by 2015SCUT ==
 
== Improvement by 2015SCUT ==
  
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'''Overview'''
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Group: 2015SCUT
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Author: Xiaomei Lin
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'''Summary'''
  
 
In the iGEM competition, teams specify, design, build, and test simple biological systems made from standard, interchangeable biological parts. Most BioBrick parts have never been characterized. And it was important to make a characterization for parts, which people could use the parameter as the experimental basis. Protein expression was a key parameter for a promoter. So in this part, we aimed at measuring the fluorescence of GFP expression which was activated by our promoter, using a plate reader.
 
In the iGEM competition, teams specify, design, build, and test simple biological systems made from standard, interchangeable biological parts. Most BioBrick parts have never been characterized. And it was important to make a characterization for parts, which people could use the parameter as the experimental basis. Protein expression was a key parameter for a promoter. So in this part, we aimed at measuring the fluorescence of GFP expression which was activated by our promoter, using a plate reader.
  
 +
'''Protocol'''
 +
 +
Please see in the experience page
 +
 +
'''Result'''
 +
 +
[[Image:2015SCUT_improvement1.png|500px|thumb|center|Figure 1: The expression of fluorescence was growing with the increasing of OD.]]
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 +
'''Discussion'''
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 +
It could be seen that M36247 was a constitutive promoter at medium strength, which could activated the expression of GFP without any inducer added. And compared with our positive control J23101, M36247 were slightly stronger.
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Revision as of 04:03, 16 September 2015

Constitutive Promoter

This part promotes constitutively at medium strength.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Improvement by 2015SCUT

Group: 2015SCUT Author: Xiaomei Lin

Summary

In the iGEM competition, teams specify, design, build, and test simple biological systems made from standard, interchangeable biological parts. Most BioBrick parts have never been characterized. And it was important to make a characterization for parts, which people could use the parameter as the experimental basis. Protein expression was a key parameter for a promoter. So in this part, we aimed at measuring the fluorescence of GFP expression which was activated by our promoter, using a plate reader.

Protocol

Please see in the experience page

Result

Figure 1: The expression of fluorescence was growing with the increasing of OD.

Discussion

It could be seen that M36247 was a constitutive promoter at medium strength, which could activated the expression of GFP without any inducer added. And compared with our positive control J23101, M36247 were slightly stronger.