Difference between revisions of "Part:BBa K1850013:Design"

 
 
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<partinfo>BBa_K1850013 short</partinfo>
 
<partinfo>BBa_K1850013 short</partinfo>
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===Design Notes===
 
===Design Notes===
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We selected <partinfo>BBa_I13453</partinfo>, an arabinose promoter, because it is a titratable promoter that allows for controlled expression of pili structural genes over a broad range of inducer concentrations.
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''fim'' was amplified with the native ribosomal binding sites, so that we could maintain the dosing of the ''fim'' subunits.
  
  
  
 
===Source===
 
===Source===
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The type I pili structural and transport genes from the ''fim'' operon were amplified from the ''E. coli'' K-12 genome.
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===References===
 
===References===

Latest revision as of 21:56, 18 September 2015

pAra - fim


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
    Illegal BamHI site found at 2715
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 998
    Illegal AgeI site found at 1029
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We selected BBa_I13453, an arabinose promoter, because it is a titratable promoter that allows for controlled expression of pili structural genes over a broad range of inducer concentrations.

fim was amplified with the native ribosomal binding sites, so that we could maintain the dosing of the fim subunits.


Source

The type I pili structural and transport genes from the fim operon were amplified from the E. coli K-12 genome.

References