Difference between revisions of "Part:BBa K1583112:Design"

 
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<partinfo>BBa_K1583112 short</partinfo>
 
<partinfo>BBa_K1583112 short</partinfo>
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<partinfo>BBa_K1583112 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1583112 SequenceAndFeatures</partinfo>
  
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===Design Notes===
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<p><h3>Design Notes</h3></p>
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The scar is a stop codon, but no terminator. Like this, cloning a GFP which contains an RBS we intended to directly couple CsgA expression to expression of GFP.
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<p>To measure the transcriptional and translational rates of CsgA production, we created this device. The beginning of the device consists of our standard parts: the rhamnose promoter and the CsgA gene. <br> However, there is no terminator behind the gene. In this manner we were able to clone the gene from the biobrick BBa_I13504 coding for GFP behind CsgA and into the same operon.</p>
  
  
  
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===Source===
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<p><h3>Source</h3></p>
  
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This part was synthesized. The rhamnose promoter was used by the iGEM 2014 TU Delft team and originally added by the iGEM12 Paris Bettencourt team. RBS and CsgA originate from E. coli K-12 MG1655
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This part was synthesized. The rhamnose promoter was used by the iGEM 2014 TU Delft team and originally added by the iGEM12 Paris Bettencourt team. RBS and CsgA originate from <i>E. coli K-12 MG1655</i>.
  
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===References===
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Revision as of 18:38, 18 September 2015

pRha + CsgA & GFP in same operon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1308

Design Notes

To measure the transcriptional and translational rates of CsgA production, we created this device. The beginning of the device consists of our standard parts: the rhamnose promoter and the CsgA gene.
However, there is no terminator behind the gene. In this manner we were able to clone the gene from the biobrick BBa_I13504 coding for GFP behind CsgA and into the same operon.

Source

This part was synthesized. The rhamnose promoter was used by the iGEM 2014 TU Delft team and originally added by the iGEM12 Paris Bettencourt team. RBS and CsgA originate from E. coli K-12 MG1655.