Difference between revisions of "Part:BBa K1723005"
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Revision as of 16:54, 15 September 2015
PAM rich URS J23117Alt promoter
PAM rich URS J23117 is a promoter made from the existing PAM rich URS J23117 promoter (BBa_K1723001) and mutated. This promoter has PAM (PAM = NGG sequence) rich Upstream Regulatory Sequence to enable the use of protein dCas9-ω (BBa_K1723000) as a gene transcription regulator when in complex with one sgRNA targeting the promoter such as: X0 (BBa_K1723006), X4 (BBa_K1723007), or X35 (BBa_K1723008).
Discover all the parts that can work with this one:
http://2015.igem.org/Team:EPF_Lausanne/Part_Collection
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 277
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 167
Illegal XhoI site found at 195 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]