Difference between revisions of "Part:BBa K1723001"

Revision as of 19:00, 16 September 2015

PAM rich URS j23117 promoter

PAM rich URS J23117 is an improvement of the J23117 (BBa_J23117) weak promoter. This promoter has PAM (PAM = NGG sequence) rich Upstream Regulatory Sequence to enable the use of protein dCas9-ω (BBa_K1723000) as a gene transcription regulator when in complex with one sgRNA targeting the promoter such as: Z0 (BBa_K1723002), Z35 (BBa_K1723003), or Z4 (BBa_K1723004).

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http://2015.igem.org/Team:EPF_Lausanne/Part_Collection

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 56
Illegal NheI site found at 79
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 43
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1723001 short</partinfo>
-PAM rich URS J23117 is a promoter made from the existing J23117 (BBa_J23117) weak promoter. This promoter has PAM (PAM = NGG sequence) rich Upstream Regulatory Sequence to enable the use of protein dCas9-&#969; (BBa_K1723000) as a gene transcription regulator when in complex with one sgRNA targeting the promoter such as: Z0 (BBa_K1723002), Z35 (BBa_K1723003), or Z4 (BBa_K1723004).
+<b>PAM rich URS J23117 is an improvement of the J23117 (BBa_J23117) weak promoter</b>. This promoter has PAM (PAM = NGG sequence) rich Upstream Regulatory Sequence to enable the use of protein dCas9-&#969; (BBa_K1723000) as a gene transcription regulator when in complex with one sgRNA targeting the promoter such as: Z0 (BBa_K1723002), Z35 (BBa_K1723003), or Z4 (BBa_K1723004).
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