Difference between revisions of "Part:BBa K1763441"

 
 
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<partinfo>BBa_K1763441 short</partinfo>
 
<partinfo>BBa_K1763441 short</partinfo>
  
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MaSp1 and MaSp2 12-mer block co-polymer under control of T7 promoter and RBS. Ratio of 1 MaSp1 to 1 MaSp2.
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MaSp1 and MaSp2 12-mer block co-polymer under control of T7 promoter and RBS. Ratio of 1 MaSp1 to 1 MaSp2. This composite part consists of the MaSp2-12 construct ([https://parts.igem.org/Part:BBa_K1763440 BBa_K1763440]) sublconed with a T7 RNA polymerase promoter and a strong RBS ([https://parts.igem.org/Part:BBa_K525998 BBa_K525998]). This construct is intended to generate the MaSp2-3 construct in high quantities in E. coli with an inducible T7 RNA polymerase system.
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===Biology===
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The T7 RNA polymerase expression system has been developed for large scale protein expression in E. coli. Strains carry an inducible T7 RNA polymerase gene incorporated into their genome. This allows very specific induction of protein expression because the T7 RNA polymerase promoter is not found in E. coli.
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A common laboratory strain for protein expression is BL21(DE3), which is what the 2015 UCLA iGEM team used in this project.
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<partinfo>BBa_K1763441 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1763441 SequenceAndFeatures</partinfo>
  
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===Usage===
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This construct should be transformed into an E. coli strain that can produce T7 RNA polymerase under induction such BL21(DE3).
  
 
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Latest revision as of 22:38, 18 September 2015

MaSp1/2[1:1]-12(T7)

MaSp1 and MaSp2 12-mer block co-polymer under control of T7 promoter and RBS. Ratio of 1 MaSp1 to 1 MaSp2. This composite part consists of the MaSp2-12 construct (BBa_K1763440) sublconed with a T7 RNA polymerase promoter and a strong RBS (BBa_K525998). This construct is intended to generate the MaSp2-3 construct in high quantities in E. coli with an inducible T7 RNA polymerase system.

Biology

The T7 RNA polymerase expression system has been developed for large scale protein expression in E. coli. Strains carry an inducible T7 RNA polymerase gene incorporated into their genome. This allows very specific induction of protein expression because the T7 RNA polymerase promoter is not found in E. coli.

A common laboratory strain for protein expression is BL21(DE3), which is what the 2015 UCLA iGEM team used in this project.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1302
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage

This construct should be transformed into an E. coli strain that can produce T7 RNA polymerase under induction such BL21(DE3).