Difference between revisions of "Part:BBa K1739000:Experience"
| Line 19: | Line 19: | ||
<!-- End of the user review template --> | <!-- End of the user review template --> | ||
<!-- DON'T DELETE --><partinfo>BBa_K1739000 EndReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_K1739000 EndReviews</partinfo> | ||
| + | |||
| + | ==Improvement by USTC 2016== | ||
| + | <br> | ||
| + | Our part is Part:<html> | ||
| + | <a href="https://parts.igem.org/Part:BBa_K2009357"> BBa_K2009357</a> | ||
| + | </html>.The SUP35NM gene we used is originally provided by Dong Men, PhD of Wuhan Insititue of Virology of Chinese Academy of Sciences, and the sequence is not quite the same as the existing sequence in the Parts Registry, for a lot of mutations have been done. The standardization of this gene is did by ourselves by adding the Biobrick prefix and suffix to its ends and doing a site mutation to eliminate the PstI cutting site inside it. Comparing to the sequence submitted by other team, our SUP35 gene is shorter, which means lower expressing pressure and the possibility to express more protein in the E.coli. | ||
Revision as of 10:12, 1 October 2016
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1739000
User Reviews
UNIQb6fd33a63311ee93-partinfo-00000000-QINU UNIQb6fd33a63311ee93-partinfo-00000001-QINU
Improvement by USTC 2016
Our part is Part:
BBa_K2009357
.The SUP35NM gene we used is originally provided by Dong Men, PhD of Wuhan Insititue of Virology of Chinese Academy of Sciences, and the sequence is not quite the same as the existing sequence in the Parts Registry, for a lot of mutations have been done. The standardization of this gene is did by ourselves by adding the Biobrick prefix and suffix to its ends and doing a site mutation to eliminate the PstI cutting site inside it. Comparing to the sequence submitted by other team, our SUP35 gene is shorter, which means lower expressing pressure and the possibility to express more protein in the E.coli.