Difference between revisions of "Part:BBa K1763433"

 
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<partinfo>BBa_K1763433 short</partinfo>
 
<partinfo>BBa_K1763433 short</partinfo>
  
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MaSp2 15 mer under control of T7 promoter RBS
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This composite part consists of the MaSp2-15 construct ([https://parts.igem.org/Part:BBa_K1763432 BBa_K1763432]) sublconed with a T7 RNA polymerase promoter and a strong RBS ([https://parts.igem.org/Part:BBa_K525998 BBa_K525998]). This construct is intended to generate the MaSp2-3 construct in high quantities in E. coli with an inducible T7 RNA polymerase system.
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===Biology===
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The T7 RNA polymerase expression system has been developed for large scale protein expression in E. coli. Strains carry an inducible T7 RNA polymerase gene incorporated into their genome. This allows very specific induction of protein expression because the T7 RNA polymerase promoter is not found in E. coli.
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A common laboratory strain for protein expression is BL21(DE3), which is what the 2015 UCLA iGEM team used in this project.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1763433 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1763433 SequenceAndFeatures</partinfo>
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===Usage===
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This construct should be transformed into an E. coli strain that can produce T7 RNA polymerase under induction such BL21(DE3).
  
  

Revision as of 22:20, 18 September 2015

MaSp2-15(T7)

This composite part consists of the MaSp2-15 construct (BBa_K1763432) sublconed with a T7 RNA polymerase promoter and a strong RBS (BBa_K525998). This construct is intended to generate the MaSp2-3 construct in high quantities in E. coli with an inducible T7 RNA polymerase system.

Biology

The T7 RNA polymerase expression system has been developed for large scale protein expression in E. coli. Strains carry an inducible T7 RNA polymerase gene incorporated into their genome. This allows very specific induction of protein expression because the T7 RNA polymerase promoter is not found in E. coli.

A common laboratory strain for protein expression is BL21(DE3), which is what the 2015 UCLA iGEM team used in this project.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1608
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage

This construct should be transformed into an E. coli strain that can produce T7 RNA polymerase under induction such BL21(DE3).