Difference between revisions of "Part:BBa K1723002:Experience"
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<h3>This picture represent the system we want to build using this promoter: a transistor like element in cell. The complex dCas9-sgRNA will bind on the promoter and activate or inhibit it.</h3>
 
<h3>This picture represent the system we want to build using this promoter: a transistor like element in cell. The complex dCas9-sgRNA will bind on the promoter and activate or inhibit it.</h3>
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<br/><br/><br/>
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<h3> Effects of BBa_K1723000 on BBa_K1723001 promoter </h3>
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https://static.igem.org/mediawiki/2015/9/97/EPF_Lausanne_RESULTS_EXPERIENCE.png
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<p>This chart present the effects of dCas9-ω (BBa_K1723000) on the PAM rich URS J23117 promoter (BBa_K1723001) when provided following sgRNAs:<br/><br/>
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[A] sgRNA Z4 (BBa_K1723003), activating<br/>
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[A/R1] sgRNA Z4 (BBa_K1723003), activating, AND sgRNA Z35 (BBa_K1723004), inhibiting<br/>
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[A/R2] sgRNA Z4 (BBa_K1723003), activating AND sgRNA Z0 (BBa_K1723002), inhibiting<br/>
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[R2] sgRNA Z0 (BBa_K1723002), inhibiting<br/>
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[R1] sgRNA Z35 (BBa_K1723004), inhibiting<br/> <br/>
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In comparison with:<br/> <br/>
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[B] apo-dCas9-ω (=no sgRNA) <br/>
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[Af] no dCas9-ω, no sgRNA<br/>
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</p>
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<br/><br/>
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<h3>This chart show that BBa_K1723002 when in complex with dCas9-ω repress the promoter PAM rich URS J23117 (BBa_K1723001). Visit our website for more informations: http://2015.igem.org/Team:EPF_Lausanne</h3>
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<br/><br/>
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<p>promoters strength were assessed using GFPmut2 reporter.</p>
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<br/><br/>
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===User Reviews===
 
===User Reviews===

Revision as of 18:00, 18 September 2015


Applications of BBa_K1723002

EPF_Lausanne_transistor.png

This picture represent the system we want to build using this promoter: a transistor like element in cell. The complex dCas9-sgRNA will bind on the promoter and activate or inhibit it.




Effects of BBa_K1723000 on BBa_K1723001 promoter

EPF_Lausanne_RESULTS_EXPERIENCE.png

This chart present the effects of dCas9-ω (BBa_K1723000) on the PAM rich URS J23117 promoter (BBa_K1723001) when provided following sgRNAs:

[A] sgRNA Z4 (BBa_K1723003), activating
[A/R1] sgRNA Z4 (BBa_K1723003), activating, AND sgRNA Z35 (BBa_K1723004), inhibiting
[A/R2] sgRNA Z4 (BBa_K1723003), activating AND sgRNA Z0 (BBa_K1723002), inhibiting
[R2] sgRNA Z0 (BBa_K1723002), inhibiting
[R1] sgRNA Z35 (BBa_K1723004), inhibiting

In comparison with:

[B] apo-dCas9-ω (=no sgRNA)
[Af] no dCas9-ω, no sgRNA



This chart show that BBa_K1723002 when in complex with dCas9-ω repress the promoter PAM rich URS J23117 (BBa_K1723001). Visit our website for more informations: http://2015.igem.org/Team:EPF_Lausanne



promoters strength were assessed using GFPmut2 reporter.




User Reviews

UNIQ9d41bae5e813eec7-partinfo-00000000-QINU UNIQ9d41bae5e813eec7-partinfo-00000001-QINU