Difference between revisions of "Part:BBa K1688002"

 
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<partinfo>BBa_K1688002 short</partinfo>
 
<partinfo>BBa_K1688002 short</partinfo>
  
The enzymes responsible for producing the mono-rhamnolipids (biosurfactant) are called RhlA and RhlB. The mono- rhamnolipids consist of a hydrophobic tail and a hydrophilic head. The RhlA gene codes for the fatty acid tail and it has been lifted and improved by iGEM10 tokyo-NoKoGen and iGEM14 Nankai from pseudomona aeruginosa. The biobrick from team Nankai BBa_K1331001 consists of gene RhlA whereas the biobrick BBa_K1688002  consist of a ribosomal binding site and a gene coding  RhlA.
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The genes responsible for producing mono-rhamnolipids (biosurfactant) are called RhlA and RhlB. The protein encoded by these two make up the subunits of rhamnosyltransferase I. The mono- rhamnolipids consist of a hydrophilic rhamnose head and a 3-(hydroxyalkanoyloxy) alkanoic acid (HAA) fatty acid tail. The RhlA gene codes for the fatty acid tail and has been lifted and improved by iGEM10 tokyo-NoKoGen and iGEM14 Nankai from pseudomona aeruginosa. The biobrick BBa_K1688002  is an assembly of RBS with BBa_K1331001.
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Revision as of 17:11, 15 September 2015

RhlA Gene

The genes responsible for producing mono-rhamnolipids (biosurfactant) are called RhlA and RhlB. The protein encoded by these two make up the subunits of rhamnosyltransferase I. The mono- rhamnolipids consist of a hydrophilic rhamnose head and a 3-(hydroxyalkanoyloxy) alkanoic acid (HAA) fatty acid tail. The RhlA gene codes for the fatty acid tail and has been lifted and improved by iGEM10 tokyo-NoKoGen and iGEM14 Nankai from pseudomona aeruginosa. The biobrick BBa_K1688002 is an assembly of RBS with BBa_K1331001.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 87
    Illegal BamHI site found at 647
    Illegal XhoI site found at 823
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 312
    Illegal BsaI.rc site found at 496