Difference between revisions of "Part:BBa K1713015:Design"

 
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<partinfo>BBa_K1713015 short</partinfo>
 
<partinfo>BBa_K1713015 short</partinfo>
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===Design Notes===
 
===Design Notes===
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planing
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The promote LuxPR is regulated by the concentration of AHL (N-Acyl homoserine lactone). Aiia is a kind of enzyme could hydrolyze AHL. LuxI is a kind of enzyme actives the synthetic of AHL. LuxI's RBS is B0034, Aiia's RBS is B0032. LVA is a degradation tag.
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Revision as of 13:06, 18 September 2015

luxpR+B0034+LuxI(-LVA)+B0032+AiiA(-LVA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 718
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 850
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The promote LuxPR is regulated by the concentration of AHL (N-Acyl homoserine lactone). Aiia is a kind of enzyme could hydrolyze AHL. LuxI is a kind of enzyme actives the synthetic of AHL. LuxI's RBS is B0034, Aiia's RBS is B0032. LVA is a degradation tag.


Source

planing

References