Difference between revisions of "Part:BBa K1696014"
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<partinfo>BBa_K1696014 short</partinfo> | <partinfo>BBa_K1696014 short</partinfo> | ||
We added a short linker after tag1(BBa_K1696013) to increase the original expression level of degraded protein. | We added a short linker after tag1(BBa_K1696013) to increase the original expression level of degraded protein. | ||
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| + | Mf-ssrA tag1 is the parental tags of all other variants that are able to be degraded by mf-Lon. In our experiment, we test the proteolysis characteristic of this tag by adding an degraded protein. However, the expression level of the protein is limited due to structural interference between mf-ssrA tag1 and degraded protein. Inspired by that, we further introduce a short linker after tag1, which can disturb the interference and make sure the protein can express in the right way. | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 14:31, 17 September 2015
Mf-ssrA Tag1+linker
We added a short linker after tag1(BBa_K1696013) to increase the original expression level of degraded protein.
Mf-ssrA tag1 is the parental tags of all other variants that are able to be degraded by mf-Lon. In our experiment, we test the proteolysis characteristic of this tag by adding an degraded protein. However, the expression level of the protein is limited due to structural interference between mf-ssrA tag1 and degraded protein. Inspired by that, we further introduce a short linker after tag1, which can disturb the interference and make sure the protein can express in the right way.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]