Difference between revisions of "Part:BBa K1789021"

 
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===Usage and Biology===
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==Usage and Biology==
  
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Indole-3-acetic acid (IAA), also known as auxin, is a plant hormone, which can promote growth and protect the soil from erosion. IAA can be synthesized through IAM pathway, originated from ''Pseudomonas savastanoi''. Two important enzymes are contained in this pathway, AKA IaaM and IaaH.
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[[File:IaaPathway.jpg]]
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The TALE1-fused IAAM and the TALE2-fused IAAH are randomly distributed in cell without the scaffold. This is the negative control group of our project,aimed at making our theory more convincing.
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==Sequence and Features==
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
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==Experimental Validation==
 
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===Functional Parameters===
 
===Functional Parameters===
 
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Revision as of 09:52, 18 September 2015

IAA prducer

This is a device which contains the sequence of the recombination of TALE1 and IAAM and the recombination of TALE2 and IAAH.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2724
    Illegal BamHI site found at 2311
    Illegal BamHI site found at 3669
    Illegal BamHI site found at 6072
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2431
    Illegal NgoMIV site found at 7088
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1304
    Illegal BsaI.rc site found at 5166
    Illegal BsaI.rc site found at 5370

Experimental Validation