Difference between revisions of "Part:BBa K1597001"

Revision as of 10:34, 15 September 2015

ThrC integration backbone for B. subtilis

Replication of this BioBrick can via integration in the ThrC locus from Bacillus subtilis .

During Bacillus subtilis transformation, the wanted DNA gets integrated in a loci present in the genomic DNA. One of these loci is the ThrC locus. This backbone is based on the plasmids pdg1664 and K823023. The AmyE loci from K823023 were replaced with the ThrC loci from pdg1664. Along with this exchange, the chloramphenicol resistance gene was replaced with erythromycin resistant gene (wich was also found in the pdg1664). This biobrick can be replicated both in Escherichia coli and in Bacillus subtilis, where it integrates into the genome.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 5126
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 5132
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 5126
Illegal BamHI site found at 5108
Illegal XhoI site found at 2503
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found at 5126
Illegal suffix found at 2
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found at 5126
Plasmid lacks a suffix.
Illegal XbaI site found at 5141
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NgoMIV site found at 2467
Illegal NgoMIV site found at 3606
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 3430

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1597001 short</partinfo>
-Replication of tour bioBrick can via integration in B. subtilis locus. One of these loci is the ThrC locus. This backbone is based on the plasmids pdg1664 and K823023. The AmyE loci from K823023 were replaced with the ThrC loci from pdg1664. Along with this exchange, the chloramphenicol resistance gene was replaced with erythromycin resistant gene (wich was also found in the pdg1664). This biobrick can be replicated both in E. coli and in B. subtilis, where it integrates into the genome.
+Replication of this BioBrick can via integration in the ThrC locus from ''Bacillus subtilis'' .
+During ''Bacillus subtilis'' transformation, the wanted DNA gets integrated in a loci present in the genomic DNA. One of these loci is the ThrC locus. This backbone is based on the plasmids pdg1664 and K823023. The AmyE loci from K823023 were replaced with the ThrC loci from pdg1664. Along with this exchange, the chloramphenicol resistance gene was replaced with erythromycin resistant gene (wich was also found in the pdg1664). This biobrick can be replicated both in ''Escherichia coli'' and in ''Bacillus subtilis'', where it integrates into the genome.
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