Difference between revisions of "Part:BBa K1789013"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1789013 short</partinfo>
 
<partinfo>BBa_K1789013 short</partinfo>
Line 17: Line 16:
 
<partinfo>BBa_K1789013 parameters</partinfo>
 
<partinfo>BBa_K1789013 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
 +
Usage:
 +
This part is scaf1 which is associated with a terminator. Scaf1 is used to bind TALE1 and TALE3 protein to make them fixed on the DNA. We create this part to make it more convenient every time we are required to add a scaffold.
 +
 +
 +
Biology:
 +
Scaf1 is a DNA scaffold which can bind TALE protein specifically to achieve the fixing of protein or enzyme. This part is scaf1 with a terminator after it.

Revision as of 08:50, 18 September 2015

SCAF1+Ter

This is a combination of scaffold and termination.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 145
    Illegal AgeI site found at 175
    Illegal AgeI site found at 205
    Illegal AgeI site found at 235
    Illegal AgeI site found at 265
    Illegal AgeI site found at 295
    Illegal AgeI site found at 325
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage: This part is scaf1 which is associated with a terminator. Scaf1 is used to bind TALE1 and TALE3 protein to make them fixed on the DNA. We create this part to make it more convenient every time we are required to add a scaffold.


Biology: Scaf1 is a DNA scaffold which can bind TALE protein specifically to achieve the fixing of protein or enzyme. This part is scaf1 with a terminator after it.