Difference between revisions of "Part:BBa K1696010"
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<partinfo>BBa_K1696010 short</partinfo> | <partinfo>BBa_K1696010 short</partinfo> | ||
− | The riboflavin biosynthesis genes ribABDEC from E. coli are overexpressed under the control of the inducible trc promoter. Enzymes encoded by the genes shown are: ribA, GTP cyclohydrolase II; ribB, 3,4-dihydroxy-2-butanone 4-phosphate synthase; ribD, fused diaminohydroxyphosphoribosylaminopyrimidine deaminase/ 5-amino-6-(5-phosphoribosylamino)uracil reductase; ribE, 6,7-dimethyl-8-ribityllumazine synthase; ribC, riboflavin synthase gene. | + | The riboflavin biosynthesis genes ribABDEC from ''E. coli'' are overexpressed under the control of the inducible trc promoter. Enzymes encoded by the genes shown are: ribA, GTP cyclohydrolase II; ribB, 3,4-dihydroxy-2-butanone 4-phosphate synthase; ribD, fused diaminohydroxyphosphoribosylaminopyrimidine deaminase/ 5-amino-6-(5-phosphoribosylamino)uracil reductase; ribE, 6,7-dimethyl-8-ribityllumazine synthase; ribC, riboflavin synthase gene. |
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+ | We construct this part based on BBa_K1696010. We change the RBS-C to a stronger one. So both riboflavin and FMN will improve. | ||
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Revision as of 17:00, 14 September 2015
Riboflavin enhancing parts
The riboflavin biosynthesis genes ribABDEC from E. coli are overexpressed under the control of the inducible trc promoter. Enzymes encoded by the genes shown are: ribA, GTP cyclohydrolase II; ribB, 3,4-dihydroxy-2-butanone 4-phosphate synthase; ribD, fused diaminohydroxyphosphoribosylaminopyrimidine deaminase/ 5-amino-6-(5-phosphoribosylamino)uracil reductase; ribE, 6,7-dimethyl-8-ribityllumazine synthase; ribC, riboflavin synthase gene.
We construct this part based on BBa_K1696010. We change the RBS-C to a stronger one. So both riboflavin and FMN will improve.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3386
Illegal BamHI site found at 1370
Illegal XhoI site found at 64 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 262
Illegal AgeI site found at 992
Illegal AgeI site found at 1034
Illegal AgeI site found at 2752
Illegal AgeI site found at 3139 - 1000COMPATIBLE WITH RFC[1000]