Difference between revisions of "Part:BBa K1668008"

Revision as of 13:11, 14 September 2015

tcdA1-device

the part tcdA1 device is composed of arabinose inducible promoter pBad BBa_I0500, toxin protein tcdA1 coding sequnceBBa_K1668008 and composite part mCherry BBa_K1668011.

We use the device to tandem express toxic protein tcdA1 and mCherry. Toxic protein tcdA1 is a macro channel forming toxin used for termite control in our project and mCherry is a reporter.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1205
Illegal NheI site found at 8257
Illegal NheI site found at 8590
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1651
Illegal BamHI site found at 1144
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 7691
Illegal NgoMIV site found at 8351
Illegal AgeI site found at 979
Illegal AgeI site found at 3527
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 961

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1668008 short</partinfo>
-the part tcdA1 device is composed of arabinose inducible promoter pBad [https://parts.igem.org/Part: BBa_I0500], toxin protein tcdA1 coding sequnce[https://parts.igem.org/Part: BBa_K1668008] and composite part mCherry [https://parts.igem.org/Part: BBa_K1668011]. We use the device to tandem express toxic protein tcdA1 and mCherry. Toxic protein is used for termite control in our project and mCherry is a reporter.
+the part tcdA1 device is composed of arabinose inducible promoter pBad [https://parts.igem.org/Part: BBa_I0500], toxin protein tcdA1 coding sequnce[https://parts.igem.org/Part: BBa_K1668008] and composite part mCherry [https://parts.igem.org/Part: BBa_K1668011].
+We use the device to tandem express toxic protein tcdA1 and mCherry. Toxic protein tcdA1 is a macro channel forming toxin used for termite control in our project and mCherry is a reporter.
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