Difference between revisions of "Part:BBa K1668008"
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<partinfo>BBa_K1668008 short</partinfo> | <partinfo>BBa_K1668008 short</partinfo> | ||
| − | the part tcdA1 device is composed of arabinose inducible promoter pBad [https://parts.igem.org/Part: BBa_I0500], toxin protein tcdA1 coding sequnce and composite part mCherry [https://parts.igem.org/Part: BBa_K1668011]. We use the device to tandem express toxic protein tcdA1 and mCherry. Toxic protein is used for termite control in our project and mCherry is a reporter. | + | the part tcdA1 device is composed of arabinose inducible promoter pBad [https://parts.igem.org/Part: BBa_I0500], toxin protein tcdA1 coding sequnce[https://parts.igem.org/Part: BBa_K1668008] and composite part mCherry [https://parts.igem.org/Part: BBa_K1668011]. We use the device to tandem express toxic protein tcdA1 and mCherry. Toxic protein is used for termite control in our project and mCherry is a reporter. |
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Revision as of 13:08, 14 September 2015
tcdA1-device
the part tcdA1 device is composed of arabinose inducible promoter pBad BBa_I0500, toxin protein tcdA1 coding sequnceBBa_K1668008 and composite part mCherry BBa_K1668011. We use the device to tandem express toxic protein tcdA1 and mCherry. Toxic protein is used for termite control in our project and mCherry is a reporter.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
Illegal NheI site found at 8257
Illegal NheI site found at 8590 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1651
Illegal BamHI site found at 1144 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 7691
Illegal NgoMIV site found at 8351
Illegal AgeI site found at 979
Illegal AgeI site found at 3527 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961