Difference between revisions of "Part:BBa K1732006"

Revision as of 06:37, 14 September 2015

pSB1C3-J23100-B0034-Firefly-B0015

J23100-B0034-Firefly-B0015

J23100 Promoter (BBa_J23100) with RBS (BBa_B0034), Firefly Luciferase Sequence (BBa_I712019), and B0015 Terminator (BBa_B0015).

Firefly Luciferase, also known as FLuc, is a luciferase from Photinus pyralis. It is a protein that emits light when reacted with the appropriate substrate luciferin. It is able to act as a report protein when linked to other promoters of proteins. The luminescence generated can also be used to measure the luminescence of standard luminometers and compare it to the measurements by DIY luminometers.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 465
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 370
Illegal AgeI site found at 1640
1000
COMPATIBLE WITH RFC[1000]

This sequence was codon optimized to allow maximal amounts of proteins to be created and extracted.

@@ Line 3: / Line 3: @@
 J23100-B0034-Firefly-B0015
+J23100 Promoter (BBa_J23100) with RBS (BBa_B0034), Firefly Luciferase Sequence (BBa_I712019), and B0015 Terminator (BBa_B0015).
+Firefly Luciferase, also known as FLuc, is a luciferase from Photinus pyralis. It is a protein that emits light when reacted with the appropriate substrate luciferin. It is able to act as a report protein when linked to other promoters of proteins. The luminescence generated can also be used to measure the luminescence of standard luminometers and compare it to the measurements by DIY luminometers.
 <!-- Add more about the biology of this part here
@@ Line 19: / Line 23: @@
 [[File:Example11.jpg]]
+This sequence was codon optimized to allow maximal amounts of proteins to be created and extracted.
 [[File:kenneth.jpg]]