Difference between revisions of "Part:BBa K1777000:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | We use | + | We use primer annealing to synthesize the mir-21 binding domain with Xho I cohesive end and Pme I blunt end. Cut the psiCHECK2 vector with the same two enzymes, then use T4 DNA ligase to construct the plasmid.<br> |
+ | In order to make sure of this reporter's efficiency, we synthesize two binding sites which are combined together. | ||
===Source=== | ===Source=== | ||
− | Renilla | + | Renilla Luciferase and Firefly Luciferase,as the control insert of this part both come from psiCHECK-2 Vector. |
===References=== | ===References=== | ||
+ | siCHECKTM Vectors INSTRUCTIONS FOR USE OF PRODUCTS C8011 AND C8021.(Promega)<br> | ||
+ | Dual-Luciferase® Reporter Assay System Instructions for use of Products E1910 and E1960.(Progema) |
Revision as of 08:37, 20 September 2015
two mir-21 binding sites
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We use primer annealing to synthesize the mir-21 binding domain with Xho I cohesive end and Pme I blunt end. Cut the psiCHECK2 vector with the same two enzymes, then use T4 DNA ligase to construct the plasmid.
In order to make sure of this reporter's efficiency, we synthesize two binding sites which are combined together.
Source
Renilla Luciferase and Firefly Luciferase,as the control insert of this part both come from psiCHECK-2 Vector.
References
siCHECKTM Vectors INSTRUCTIONS FOR USE OF PRODUCTS C8011 AND C8021.(Promega)
Dual-Luciferase® Reporter Assay System Instructions for use of Products E1910 and E1960.(Progema)