Difference between revisions of "Part:BBa K1777000:Design"
(→Design Notes) |
|||
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | We use PCR to synthesize the mir-21 binding domain. In order to make sure of this reporter's efficiency, we synthesize two binding sites which are combined together. | + | We use overlap PCR to synthesize the mir-21 binding domain. In order to make sure of this reporter's efficiency, we synthesize two binding sites which are combined together. |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
Revision as of 17:04, 13 September 2015
two mir-21 binding sites
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We use overlap PCR to synthesize the mir-21 binding domain. In order to make sure of this reporter's efficiency, we synthesize two binding sites which are combined together.
Source
Renilla luciferase ofthis part come from psiCHECK-2 Vector.