Difference between revisions of "Part:BBa K1766008"

 
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<partinfo>BBa_K1766008 short</partinfo>
 
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EnvZ is part of the EnvZ-OmpR two-component regulatory system for controlling osmotic pressure in <i>E.coli</i>. Structure includes a C-terminal  V5-tag through a GGSSAAG linker. The part was used in a pRD400 backbone behind a lac-inducible promoter to study signaling as well as expression.  
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EnvZ is part of the EnvZ-OmpR two-component regulatory system for controlling osmotic pressure in <i>E.coli</i>. Structure includes the EnvZ247 allele [1] which is linked through a GGSSAAG linker to a V5 epitope tag. The full structure has a predicted size of 115.36 kDa [2]. The part was provided in the pRD400 (or pEnvZ) backbone under a lac-inducible promoter [3].  
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===Usage and Biology===
 
  
 
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<partinfo>BBa_K1766008 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1766008 SequenceAndFeatures</partinfo>
  
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===Experiments===
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<b>Experssion</b>
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<br />
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<b>Osmolarity tests</b>
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<br />
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===References===
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[1] Russo, F. D. (1992) Ph.D. thesis. Princeton University, Princeton, N.J.
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<br />
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[2] Gasteiger E., Hoogland C., Gattiker A., Duvaud S., Wilkins M.R., Appel R.D., Bairoch A. (2005) <i>Protein Identification
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and Analysis Tools on the ExPASy Server</i>; (In) John M. Walker (ed): The Proteomics Protocols Handbook, Humana Press
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<br />
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[3] Nørholm M. H. H., von Heijne G., and Draheim R. R. (2014) <i>Forcing the Issue: Aromatic Tuning Facilitates Stimulus-Independent Modulation of a Two-Component Signaling Circuit</i>. ACS Synth. Biol. 2015, 4, 474−481
  
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===Functional Parameters===
 
 
<partinfo>BBa_K1766008 parameters</partinfo>
 
<partinfo>BBa_K1766008 parameters</partinfo>
 
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Revision as of 13:21, 14 September 2015

EnvZ_V5 osmoregulatory histidine kinase from E.coli.

EnvZ is part of the EnvZ-OmpR two-component regulatory system for controlling osmotic pressure in E.coli. Structure includes the EnvZ247 allele [1] which is linked through a GGSSAAG linker to a V5 epitope tag. The full structure has a predicted size of 115.36 kDa [2]. The part was provided in the pRD400 (or pEnvZ) backbone under a lac-inducible promoter [3].


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 316
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 316
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 316
    Illegal BglII site found at 236
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 316
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 316
  • 1000
    COMPATIBLE WITH RFC[1000]

Experiments

Experssion

Osmolarity tests


References

[1] Russo, F. D. (1992) Ph.D. thesis. Princeton University, Princeton, N.J.
[2] Gasteiger E., Hoogland C., Gattiker A., Duvaud S., Wilkins M.R., Appel R.D., Bairoch A. (2005) Protein Identification and Analysis Tools on the ExPASy Server; (In) John M. Walker (ed): The Proteomics Protocols Handbook, Humana Press
[3] Nørholm M. H. H., von Heijne G., and Draheim R. R. (2014) Forcing the Issue: Aromatic Tuning Facilitates Stimulus-Independent Modulation of a Two-Component Signaling Circuit. ACS Synth. Biol. 2015, 4, 474−481