Difference between revisions of "Part:BBa K1632013:Experience"
(→Materials and Methods) |
(→Materials and Methods) |
||
| Line 3: | Line 3: | ||
All the samples were DH5α strain with antibiotic resistance to ampicillin and kanamycin.<br> | All the samples were DH5α strain with antibiotic resistance to ampicillin and kanamycin.<br> | ||
| − | A. PBAD/araC_FimE( | + | A. PBAD/araC_FimE(6A1) +Fimswitch[default ON]_gfp(3K3) <br> |
| − | B. PBAD/araC_FimE( | + | B. PBAD/araC_FimE(6A1) +Fimswitch[default ON]_gfp(3K3) <br> |
C. rbs_M256IcysE(6A1) + Fimswitch[ON]_gfp(3K3) …positive control 1<br> | C. rbs_M256IcysE(6A1) + Fimswitch[ON]_gfp(3K3) …positive control 1<br> | ||
D. rbs_M256IcysE(6A1) + Fimswitch[OFF]_gfp(3K3) …negative control 1<br> | D. rbs_M256IcysE(6A1) + Fimswitch[OFF]_gfp(3K3) …negative control 1<br> | ||
Revision as of 13:42, 13 September 2015
Materials and Methods
1. Construction
All the samples were DH5α strain with antibiotic resistance to ampicillin and kanamycin.
A. PBAD/araC_FimE(6A1) +Fimswitch[default ON]_gfp(3K3)
B. PBAD/araC_FimE(6A1) +Fimswitch[default ON]_gfp(3K3)
C. rbs_M256IcysE(6A1) + Fimswitch[ON]_gfp(3K3) …positive control 1
D. rbs_M256IcysE(6A1) + Fimswitch[OFF]_gfp(3K3) …negative control 1
E. PBAD/araC_FimE(6A1) + J23119_gfp(3K3) …positive control 2
F. PBAD/araC_FimE(6A1)+rbs_gfp(3K3) …negative control 2
2. Assay protocol