Difference between revisions of "Part:BBa K1632011:Experience"

Revision as of 13:29, 13 September 2015

Materials and Methods

1. Construction
All the samples were DH5α strain with antibiotic resistance to ampicillin and kanamycin.

A. PBAD/araC_FimE(4A5) +Fimswitch[default ON]_gfp(3K3)
B. PBAD/araC_FimE(4A5) +Fimswitch[default ON]_gfp(3K3)
C. rbs_M256IcysE(6A1) + Fimswitch[ON]_gfp(3K3) …positive control 1
D. rbs_M256IcysE(6A1) + Fimswitch[OFF]_gfp(3K3) …negative control 1
E. PBAD/araC_FimE(6A1) + J23119_gfp(3K3) …positive control 2
F. PBAD/araC_FimE(6A1)+rbs_gfp(3K3) …negative control 2

2. Assay protocol

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 A. PBAD/araC_FimE(4A5) +Fimswitch[default ON]_gfp(3K3) <br>
 B. PBAD/araC_FimE(4A5) +Fimswitch[default ON]_gfp(3K3) <br>
-C. no gene(4A5) + Fimswitch[ON]_gfp(3K3) …positive control 1<br>
+C. rbs_M256IcysE(6A1) + Fimswitch[ON]_gfp(3K3) …positive control 1<br>
-D. no gene(4A5) + Fimswitch[OFF]_gfp(3K3) …negative control 1<br>
+D. rbs_M256IcysE(6A1) + Fimswitch[OFF]_gfp(3K3) …negative control 1<br>
 E. PBAD/araC_FimE(6A1) + J23119_gfp(3K3) …positive control 2 <br>
 F. PBAD/araC_FimE(6A1)+rbs_gfp(3K3) …negative control 2 <br>
 <b>2. Assay protocol</b><br>