Difference between revisions of "Part:BBa K1699005:Design"

(→‎References)
(→‎Design Notes)
Line 7: Line 7:
 
===Design Notes===
 
===Design Notes===
  
−
gRNA in SaCas9 scaffold under U6 promoter. Designed to complement 3 sites on MLP synthetic promoter.
+
Designed using Benchling to meet registry standards. Synthesized by IDT. Cloned into pSB1C3 using EcoRI and PstI restrictoion sites.
−
<br /> Cloned into pSBC13 using EcoRI and PstI restriction sites.
+
−
<br /> F. tagtcatggcggccgcgtcgacG
+
−
<br /> R. actgacatgcggccgcttaattaaC
+
  
 
===Source===
 
===Source===
  
 
gMLP from IDT de-novo synthesis. Cloning with U6 to pSBC13 was done by team BGU 2015.
 
gMLP from IDT de-novo synthesis. Cloning with U6 to pSBC13 was done by team BGU 2015.

Revision as of 11:35, 13 September 2015

gRNA for dCas9-VP64 targeting synthetic activation promoter pMLPm under U6 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 250
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designed using Benchling to meet registry standards. Synthesized by IDT. Cloned into pSB1C3 using EcoRI and PstI restrictoion sites.

Source

gMLP from IDT de-novo synthesis. Cloning with U6 to pSBC13 was done by team BGU 2015.