Difference between revisions of "Part:BBa K1633003:Experience"

Revision as of 11:02, 18 September 2015

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1633003

INTRODUCTION

This part is an artificially designed RNA strand. It serves as an element of the Team NJU-CHINA RNAi module. We use it as the siRNA medicine to downregulate the expression of Mu opioid receptor in brain tissue. We designed specific MOR siRNAs based on a free software accessible online. This tool can find the best siRNA sequences on target gene MOR to insure the maximum gene-specificity and silencing efficacy. This tool also designs the pair of oligonucleotides needed to generate short hairpin RNAs (shRNAs) in the plasmid. Then we synthesize the shRNA sequences with the help of a DNA synthesis company. We totally got four such shRNA plasmids.Although MOR siRNA-1 plasmid can efficiently knock down MOR expression, it does not show best interference efficiency and therefore serve as a backup.

User Reviews

UNIQ867e3ff29c2ac08c-partinfo-00000000-QINU UNIQ867e3ff29c2ac08c-partinfo-00000001-QINU

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 ===Applications of BBa_K1633003===
+'''INTRODUCTION'''
+This part is an artificially designed RNA strand. It serves as an element of the Team NJU-CHINA RNAi module. We use it as the siRNA medicine to downregulate the expression of Mu opioid receptor in brain tissue. We designed specific MOR siRNAs based on a free software accessible online. This tool can find the best siRNA sequences on target gene MOR to insure the maximum gene-specificity and silencing efficacy. This tool also designs the pair of oligonucleotides needed to generate short hairpin RNAs (shRNAs) in the plasmid. Then we synthesize the shRNA sequences with the help of a DNA synthesis company. We totally got four such shRNA plasmids.Although MOR siRNA-1 plasmid can efficiently knock down MOR expression, it does not show best interference efficiency and therefore serve as a backup.
+[[File:Example.jpg]]
 ===User Reviews===