Difference between revisions of "Part:BBa K1714001:Design"
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===Design Notes===
 
===Design Notes===
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There's no design considerations.  
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ABF-2 is under control of inducible promoters; Plac promoter. In the absence of IPTG, lacI binds to lac operator region and represses the transcription, therefore expression of gene downstream of Plac is negatively controlled. By adding IPTG, this inducer interacts with lacI and changes lacI's conformation.. Finally, lacI is released from promoter region, leading recruiting of RNA polymerase and initiation of transcription. We predict that E. coli could be difficult to grow due to leak of Plac (Even if we don’t activated Plac by IPTG, gene downstream would be transcribed a little and thanatin could kill host cells) if this construct has never mutated.  
  
  

Revision as of 16:17, 18 September 2015

pLac-RBS B0034-ABF-2-double terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 321
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

ABF-2 is under control of inducible promoters; Plac promoter. In the absence of IPTG, lacI binds to lac operator region and represses the transcription, therefore expression of gene downstream of Plac is negatively controlled. By adding IPTG, this inducer interacts with lacI and changes lacI's conformation.. Finally, lacI is released from promoter region, leading recruiting of RNA polymerase and initiation of transcription. We predict that E. coli could be difficult to grow due to leak of Plac (Even if we don’t activated Plac by IPTG, gene downstream would be transcribed a little and thanatin could kill host cells) if this construct has never mutated.


Source

This part's CDS's http://www.ncbi.nlm.nih.gov/gene/266826


References

http://www.ncbi.nlm.nih.gov/pubmed/23945047