Difference between revisions of "Part:BBa K1686047:Design"

 
 
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<partinfo>BBa_K1686047 short</partinfo>
 
<partinfo>BBa_K1686047 short</partinfo>
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===Design Notes===
 
===Design Notes===
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The sequence has been modified for codon optimization for E.coli.
 
  
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The gene of CrdC comes from ''Agrobacterium sp.'' ATCC31749 and we would like to use it in ''E.coli'', so we fixed the bias codon by using the software of ''Integrated DNA Technologies, Inc'' for codon optimization.
  
  
 
===Source===
 
===Source===
  
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AECL01000028.1
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The nucleotide sequence of CrdC comes from GenBank under the accession number AECL01000028.1
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===References===
 
===References===
 +
 +
Stasinopoulos SJ, et al. (1999). "Detection of two loci involved in (1→3)-β-glucan (curdlan) biosynthesis by Agrobacterium sp. ATCC31749, and comparative sequence analysis of the putative curdlan synthase gene." Glycobiology 9:31–41.
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'''

Latest revision as of 15:53, 12 September 2015

crdC gene with codon optimisation for E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 361
    Illegal AgeI site found at 601
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The gene of CrdC comes from Agrobacterium sp. ATCC31749 and we would like to use it in E.coli, so we fixed the bias codon by using the software of Integrated DNA Technologies, Inc for codon optimization.


Source

The nucleotide sequence of CrdC comes from GenBank under the accession number AECL01000028.1


References

Stasinopoulos SJ, et al. (1999). "Detection of two loci involved in (1→3)-β-glucan (curdlan) biosynthesis by Agrobacterium sp. ATCC31749, and comparative sequence analysis of the putative curdlan synthase gene." Glycobiology 9:31–41.