Difference between revisions of "Part:BBa K1686046:Design"
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===Design Notes===
 
===Design Notes===
  
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The gene of Curdlan synthase comes from Agrobacterium and we would like to use it in ''E.coli'', so we fixed the bias codon by using the software of ''Integrated DNA Technologies, Inc'' for codon optimization.
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The gene of Curdlan synthase comes from ''Agrobacterium sp.'' ATCC31749 and we would like to use it in ''E.coli'', so we fixed the bias codon by using the software of ''Integrated DNA Technologies, Inc'' for codon optimization.
  
  

Revision as of 13:40, 12 September 2015

Curdlan synthase gene with codon optimisation for E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 678
    Illegal XhoI site found at 400
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 801
    Illegal AgeI site found at 1192
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The gene of Curdlan synthase comes from Agrobacterium sp. ATCC31749 and we would like to use it in E.coli, so we fixed the bias codon by using the software of Integrated DNA Technologies, Inc for codon optimization.


Source

The nucleotide sequence of crdS comes from GenBank under the accession number AF057142.2


References

Stasinopoulos SJ, et al. (1999). "Detection of two loci involved in (1→3)-β-glucan (curdlan) biosynthesis by Agrobacterium sp. ATCC31749, and comparative sequence analysis of the putative curdlan synthase gene." Glycobiology 9:31–41.