Difference between revisions of "Part:BBa K1641023"

(Created page with "This is a reporter of invertase activity of Cre for Real-time measurement of dynamics of timing modules by SYSU-CHINA 2015. The target sequence LoxP of Cre locates in the pInv-...")
 
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__NOTOC__
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<partinfo>BBa_K1641023 short</partinfo>
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This is a reporter of invertase activity of Cre for Real-time measurement of dynamics of timing modules by SYSU-CHINA 2015.  
 
This is a reporter of invertase activity of Cre for Real-time measurement of dynamics of timing modules by SYSU-CHINA 2015.  
  
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mcherry type: BBa_J06504-BBa_M0052, which is mcherry-ssra(moderately fast)
 
mcherry type: BBa_J06504-BBa_M0052, which is mcherry-ssra(moderately fast)
 
Promoter type: BBa_J23101
 
Promoter type: BBa_J23101
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1641023 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K1641023 parameters</partinfo>
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Revision as of 09:45, 12 September 2015

Reporter of Invertase activity of Cre, pInv-rep-101LoxM

This is a reporter of invertase activity of Cre for Real-time measurement of dynamics of timing modules by SYSU-CHINA 2015.

The target sequence LoxP of Cre locates in the pInv-rep, surrounding a mcherry gene which is yet upside-down and transcribed by a constructive promoter. This mcherry-coding sequence can be inverted and restored to 5’ – 3’ direction at the existence of Cre or its EGFP fusion, rendering red signal. By real-time measurement of EGFP and mcherry, we can obtain data of Cre dynamics and simulate this process through modelling.

For this reporter: RTS type: LoxP mcherry type: BBa_J06504-BBa_M0052, which is mcherry-ssra(moderately fast) Promoter type: BBa_J23101

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]