Difference between revisions of "Part:BBa K1813063"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1813063 short</partinfo> | <partinfo>BBa_K1813063 short</partinfo> | ||
ptac pelB AgCPR pelb HUMCYPDB1 | ptac pelB AgCPR pelb HUMCYPDB1 | ||
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+ | With ptac powering the transcription of <html><b> <a href="https://parts.igem.org/Part:BBa_K1813053">BBa_K1813053</a></b></html> and <html><b> <a href="https://parts.igem.org/Part:BBa_K1813055">BBa_K1813055</a></b></html>, this part allows the co-localization of large amounts of simultaneously expressed HUMCYPDB1 (<html><b> <a href="https://parts.igem.org/Part:BBa_K1813005">BBa_K1813005</a></b></html>) and <i>A. gambiae</i> NADPH p450 reductase (<html><b> <a href="https://parts.igem.org/Part:BBa_K1813010">BBa_K1813010</a></b></html>) to the bacterial periplasm. | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 12:19, 18 September 2015
ptac pelB AgCPR pelb CYP2D6
ptac pelB AgCPR pelb HUMCYPDB1
With ptac powering the transcription of BBa_K1813053 and BBa_K1813055, this part allows the co-localization of large amounts of simultaneously expressed HUMCYPDB1 ( BBa_K1813005) and A. gambiae NADPH p450 reductase ( BBa_K1813010) to the bacterial periplasm.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2783
Illegal BamHI site found at 626 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 141
Illegal NgoMIV site found at 2448
Illegal NgoMIV site found at 3373
Illegal AgeI site found at 424
Illegal AgeI site found at 1606
Illegal AgeI site found at 1762 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2122