Difference between revisions of "Part:BBa K1696007"

 
 
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The trans-acting protein RcfB, however, is involved in basal activity of P170 and is also essential for pH induction. The protein RcfB belongs to the Crp-Fnr family of transcription regulators, which, upon activation, bind a DNA recognition motif within a promoter region and activates transcription.
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The trans-acting protein RcfB, which belongs to the Crp-Fnr family of transcription regulators, is involved in basal activity of P170 and is also essential for pH induction. To our knowledge, no Crp-Fnr protein is known to respond to an acid signal, so it is an remarkable finding that RcfB is activated when the cells are exposed to acid environments. The protein RcfB contains conserved glycine residues, activating regions 1 and 3, D α-helix, and C-terminal helix-turn-helix (HTH) DNA binding motif, which, upon activation, bind a DNA recognition motif within a promoter region and activates transcription.
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== Reference ==
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Søren M. Madsen, Thomas Hindré, Jean-Paul Le Pennec, et al. Two acid-inducible promoters from Lactococcus lactis require the cis-acting ACiD-box and the transcription regulator RcfB[J]. Molecular Microbiology, 2005, 56(3):735-746.
  
 
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Latest revision as of 14:11, 17 September 2015

RcfB

The trans-acting protein RcfB, which belongs to the Crp-Fnr family of transcription regulators, is involved in basal activity of P170 and is also essential for pH induction. To our knowledge, no Crp-Fnr protein is known to respond to an acid signal, so it is an remarkable finding that RcfB is activated when the cells are exposed to acid environments. The protein RcfB contains conserved glycine residues, activating regions 1 and 3, D α-helix, and C-terminal helix-turn-helix (HTH) DNA binding motif, which, upon activation, bind a DNA recognition motif within a promoter region and activates transcription.


Reference

Søren M. Madsen, Thomas Hindré, Jean-Paul Le Pennec, et al. Two acid-inducible promoters from Lactococcus lactis require the cis-acting ACiD-box and the transcription regulator RcfB[J]. Molecular Microbiology, 2005, 56(3):735-746.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]