Difference between revisions of "Part:BBa K1696004"

Latest revision as of 14:03, 17 September 2015

L-lactate producing part2

The NAD+-dependent L-lactate dehydrogenase gene from Geobacillus stearothermophilus is a promising enzyme for L-lactate mass production. In order to achieve even more lactate generation to feed exoelectrogen in our system, we choose a strong promoter and strong RBS to construct L-Lactate part. Considering that the strong promoter and RBS can ensure the over-expression of L-lactate, we believe that this kind of construction will lead to an increasing power generation and potential application in the future.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 248
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 36
Illegal BsaI.rc site found at 1028

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1696004 short</partinfo>
-We choose a strong promoter+ strong RBS to construct L-Lactate part. The NAD+-dependent L-lactate dehydrogenase gene is from Geobacillus stearothermophilus.
+The NAD+-dependent L-lactate dehydrogenase gene from ''Geobacillus stearothermophilus'' is a promising enzyme for L-lactate mass production. In order to achieve even more lactate generation to feed exoelectrogen in our system, we choose a strong promoter and strong RBS to construct L-Lactate part. Considering that the strong promoter and RBS can ensure the over-expression of L-lactate, we believe that this kind of construction will lead to an increasing power generation and potential application in the future.