Difference between revisions of "Part:BBa K1696002"

 
 
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L-Lactic acid, one of the most important chiral molecules and organic acids, is produced via pyruvate from carbohydrates in diverse microorganisms catalyzed by an NAD+-dependent L-lactate dehydrogenase. Naturally, Escherichia coli does not produce L-lactate in noticeable amounts, but can catabolize it via a dehydrogenation reaction mediated by an FMN-dependent L-lactate dehydrogenase.
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Enzymes have become important tools in several industries due to their ability to produce chirally pure and complex molecules with interesting biological properties. The NAD(+)-dependent LDH (lactate dehydrogenase, bsLDH) from ''G. stearothermophilus'' (formerly ''Bacillus stearothermophilus'') is a particularly crucial enzyme in the pharmaceutical industry and is related to other metabolites in terms of NADH use and regeneration. LDH catalyses the interconversion of pyruvate and lactate using the NADH/NAD(+) pair as a redox cofactor.
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== References:==
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[1] N G, Karagüler, R B, Sessions, B, Binay, et al. Protein engineering applications of industrially exploitable enzymes: Geobacillus stearothermophilus LDH and Candida methylica FDH.[J]. Biochem Soc Trans, 2007, 35(Pt 6):1610-1615.
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Latest revision as of 13:51, 17 September 2015

L-Lactate producing module from Geobacillus stearothermophilus


Enzymes have become important tools in several industries due to their ability to produce chirally pure and complex molecules with interesting biological properties. The NAD(+)-dependent LDH (lactate dehydrogenase, bsLDH) from G. stearothermophilus (formerly Bacillus stearothermophilus) is a particularly crucial enzyme in the pharmaceutical industry and is related to other metabolites in terms of NADH use and regeneration. LDH catalyses the interconversion of pyruvate and lactate using the NADH/NAD(+) pair as a redox cofactor.


References:

[1] N G, Karagüler, R B, Sessions, B, Binay, et al. Protein engineering applications of industrially exploitable enzymes: Geobacillus stearothermophilus LDH and Candida methylica FDH.[J]. Biochem Soc Trans, 2007, 35(Pt 6):1610-1615.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 180
  • 1000
    COMPATIBLE WITH RFC[1000]