Difference between revisions of "Part:BBa K1640012:Design"
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<partinfo>BBa_K1640012 short</partinfo> | <partinfo>BBa_K1640012 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
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− | + | Genes required for photosystem II were selected using <html><a href=http://www.biocyc.org/CHLAMY/NEW-IMAGE?type=ENZYME&object=CPLX4LZ-235>biocyc entry on <i>Chlamydomonas reinhardtii</i> photosystem II complex</a>. Sequences were obtained from NCBI, targeting sequences were identified in nuclear using <a href=http://www.cbs.dtu.dk/services/ChloroP>ChloroP</a>, and removed, with an ATG added. Resulting sequences were codon optimized for e. coli expression using <a href=http://genomes.urv.es/OPTIMIZER/>Optimizer</a></html>, avoiding EcoRI, XbaI, SpeI and XbaI sites. | |
− | + | Ribosome binding sites were added in front of each CDS, and a promoter was added to biobricks which form the first part of their intended operon. |
Latest revision as of 08:41, 18 September 2015
psbQR
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 451
Illegal BglII site found at 780 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 844
Design Notes
Genes required for photosystem II were selected using biocyc entry on Chlamydomonas reinhardtii photosystem II complex. Sequences were obtained from NCBI, targeting sequences were identified in nuclear using ChloroP, and removed, with an ATG added. Resulting sequences were codon optimized for e. coli expression using Optimizer, avoiding EcoRI, XbaI, SpeI and XbaI sites.
Ribosome binding sites were added in front of each CDS, and a promoter was added to biobricks which form the first part of their intended operon.