Difference between revisions of "Part:BBa K1641005"

Latest revision as of 09:09, 10 September 2015

Fusion protein of Flp-EGFP-ssra, with RBS at beginning

This is a sequence of RBS (BBa_B0034) followed by a fusion protein of Flp-EGFP-ssra. This part is for expression of Flp-EGFP-ssra, which aims to overturn our invertase modules in our Micro-timer.

Flp is a commonly used recombinase originated from yeast, with invertase activity that turns the DNA sequence between two anti-parallel FRT sites up-side-down. A EGFP fusion at the C-term of Flp will not significantly interfere the enzymatic activity, and a flexible chain of 8 AA is added between the two part to further avoid side-effect like misfolding. A ssra-tag (BBa_M0051) can accelerate the degradation of the protein, in order to clean it up when not in need.

Note: The brick sequence starts directly after the XbaI of prefix and ends before SpeI of the Suffix, without the useless bases (G and T respectively) added before and after brick sequence. The scar will be "ACTAGA" safely without cutting activity and start code. with This deletion do not interfere the usage of the brick.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 522
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1641005 short</partinfo>
-This is a sequence of RBS (BBa_B0034) followed by a fusion protein of Flp::EGFP::ssra-tag. This part is for expression of Flp::EGFP::ssra-tag, which aims to overturn our invertase modules in our Micro-timer.
+This is a sequence of RBS (BBa_B0034) followed by a fusion protein of Flp-EGFP-ssra. This part is for expression of Flp-EGFP-ssra, which aims to overturn our invertase modules in our Micro-timer.
 Flp is a commonly used recombinase originated from yeast, with invertase activity that turns the DNA sequence between two anti-parallel FRT sites up-side-down. A EGFP fusion at the C-term of Flp will not significantly interfere the enzymatic activity, and a flexible chain of 8 AA is added between the two part to further avoid side-effect like misfolding. A ssra-tag (BBa_M0051) can accelerate the degradation of the protein, in order to clean it up when not in need.
+Note: The brick sequence starts directly after the XbaI of prefix and ends before SpeI of the Suffix, without the useless bases (G and T respectively) added before and after brick sequence. The scar will be "ACTAGA" safely without cutting activity and start code. with This deletion do not interfere the usage of the brick.