Difference between revisions of "Part:BBa K1597000"

Revision as of 10:30, 15 September 2015

Salt inducible promoter for B. subtilis

Bacillus subtilis is capable of coping with fluctuating salt concentrations. One of the genes involved is proH, this is an 1-pyrroline-5-caboxylate reductase. A study has shown that this gene is under control of the proH-promoter. The proH promoter can be activated by a range of salt concentrations, respectively ranging from 0,1M salt up to 1 M. We have cloned this promoter out of the genome of Bacillus subtilis and combined it with tasA (a gene used in forming the amyloid-like fibers of the biofilm matrix) and GFP. The latter was used to validate the promoter.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 48
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K1597000 short</partinfo>
-Bacillus subtilis is capable to cope with fluctuating salt concentrations. One of the genes involved is the proH, this is an 1-pyrroline-5-caboxylate reductase. A studie has shown that this gene is under control of  the proH-promoter wich can be activated by a range of salt concentrations.
+''Bacillus subtilis'' is capable of coping with fluctuating salt concentrations. One of the genes involved is proH, this is an 1-pyrroline-5-caboxylate reductase. A study has shown that this gene is under control of the proH-promoter. The proH promoter can be activated by a range of salt concentrations, respectively ranging from 0,1M salt up to 1 M.
+We have cloned this promoter out of the genome of ''Bacillus subtilis'' and combined it with tasA (a gene used in forming the amyloid-like fibers of the biofilm matrix) and GFP. The latter was used to validate the promoter.
-We have cloned this promoter out of the genome of bacillus and combined it with TasA (an amyloid producing gene) and GFP. The latter was used to validate the promoter.
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