Difference between revisions of "Part:BBa K1618000"
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<partinfo>BBa_K1618000 short</partinfo>
 
<partinfo>BBa_K1618000 short</partinfo>
  
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GlgB codes for an enzyme that increases glycogen branching in glycogen. The enzyme cleaves alpha-1,4 linkages in glycogen and transfers the cleaved branch back onto the main glycogen strand using an alpha-1,6 linkage. This creates the branch. The genetic sequence is inserted into the pSB1C3 and therefore displays chloramphenicol resistance.  
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BBa_K1618000 contains the coding sequence for GlgB from <i>E. coli</i>. GlgB cleaves alpha-1,4 linkages in glycogen chains and transfers the cleaved branch onto the main glycogen chain using an alpha-1,6 linkage to make a branch point.
  
 
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Latest revision as of 09:05, 21 September 2015

GlgB without inducible promoter

BBa_K1618000 contains the coding sequence for GlgB from E. coli. GlgB cleaves alpha-1,4 linkages in glycogen chains and transfers the cleaved branch onto the main glycogen chain using an alpha-1,6 linkage to make a branch point.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 57
    Illegal BamHI site found at 1265
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 691