Difference between revisions of "Part:BBa K1773003:Design"

Revision as of 12:40, 9 September 2015

I-F type CRISPR-Cas Cas3 gene

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 2623
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1344
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 2307
Illegal AgeI site found at 2680
1000
COMPATIBLE WITH RFC[1000]

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(Restriction with EcoRI (E), XbaI (X) and PstI (P) of Wild type (WT) Cas3 gene compared to mutated Cas3 genes. The first mutant plasmid has two successful mutations, whereas the second mutant plasmid only has one successfully mutated restriction site.)

LALALALA ===Design Notes=== no ===Source=== A.actinomycetemcomitans D7-S ===References===

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-[[File:Cas3 1.png.JPG|300px|thumb|(Restriction with EcoRI (E), XbaI (X) and PstI (P) of Wild type (WT) Cas3 gene compared to mutated Cas3 genes. The first mutant plasmid has two successful mutations, whereas the second mutant plasmid only has one successfully mutated restriction site.)|left]]
+[[File:Cas3 1.JPG|300px|thumb|(Restriction with EcoRI (E), XbaI (X) and PstI (P) of Wild type (WT) Cas3 gene compared to mutated Cas3 genes. The first mutant plasmid has two successful mutations, whereas the second mutant plasmid only has one successfully mutated restriction site.)|left]]
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 LALALALA