Difference between revisions of "Part:BBa K1668002:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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We used seamless assembly.
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By PCR with primers frr1 and frr2 shown below, we added the standard prefix and suffix at both ends of the frr sequence. We used seamless assembly as our assembly method. Therefore, there are no scar between prefix sequence, frr, and suffix sequence.
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<br>
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frr1 (F, 5’-3’): GAATTCGCGGCCGCTTCTAGATGCGCGGGTACGTC
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<br>
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frr2 (R, 5’-3’): TGCAGCGGCCGCTACTAGTATTATTACATCAAGGTCGCC
  
 
===Source===
 
===Source===

Revision as of 09:44, 11 September 2015


frr (from Streptomyces avermitilis, increasing avermectin production)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 165
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 337


Design Notes

By PCR with primers frr1 and frr2 shown below, we added the standard prefix and suffix at both ends of the frr sequence. We used seamless assembly as our assembly method. Therefore, there are no scar between prefix sequence, frr, and suffix sequence.
frr1 (F, 5’-3’): GAATTCGCGGCCGCTTCTAGATGCGCGGGTACGTC
frr2 (R, 5’-3’): TGCAGCGGCCGCTACTAGTATTATTACATCAAGGTCGCC

Source

The metK gene was amplified by PCR with genomic DNA of S. avermitilis(具体型号) as template using primers

References