Difference between revisions of "Part:BBa K1732004:Design"

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(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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J23100 constitutive promoter (BBa_J23100), a RBS (BBa_B0034), CDcel domain (BBa_K1732002), Gaussia luciferase (BBa_K1732003), and B0015 terminator (BBa_B0015).
 +
 
E.coli was codon optimized for E.coli.
 
E.coli was codon optimized for E.coli.
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−
 
  
 
===Source===
 
===Source===

Revision as of 03:19, 8 September 2015


pSB1C3-J23100-B0034-CDcel-Gaussia-His-B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1517
    Illegal BamHI site found at 975
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 715


Design Notes

J23100 constitutive promoter (BBa_J23100), a RBS (BBa_B0034), CDcel domain (BBa_K1732002), Gaussia luciferase (BBa_K1732003), and B0015 terminator (BBa_B0015).

E.coli was codon optimized for E.coli.

Source

Synthesized using IDT by Cheryl Telmer from the Bruchez lab in Carnegie Mellon University.

References