Difference between revisions of "Part:BBa K1666002:Design"
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===Source=== | ===Source=== | ||
| − | We extracted the genomic DNA of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2, and used PCR to isolate the gene | + | We extracted the genomic DNA of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2, and used PCR to isolate the gene ''lsrC''(Gene ID: 1255602). |
===References=== | ===References=== | ||
Revision as of 14:46, 6 September 2015
LsrC of LuxS/AI-2 signaling pathway in Salmonalla
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 295
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The lsrC gene was isolated from the genome of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 using PCR. We added restriction enzyme sites when designing primers, and the assembly is done through the use of restriction sites (cutting and ligating).
Source
We extracted the genomic DNA of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2, and used PCR to isolate the gene lsrC(Gene ID: 1255602).