Difference between revisions of "Part:BBa K1824008"

 
 
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<partinfo>BBa_K1824008 short</partinfo>
 
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This is a composite regulatory part that consisting of T7 promoter BBa_I13453 and RNA Thermometer U6 BBa_K1824564 for temperature induced gene expression at 37 degrees. The possible secondary structure of A1 was simulated by RNAstructure (Fig.1). This combination is compatible with the assembly method that XJTLU-CHINA promoted.
 
This is a composite regulatory part that consisting of T7 promoter BBa_I13453 and RNA Thermometer U6 BBa_K1824564 for temperature induced gene expression at 37 degrees. The possible secondary structure of A1 was simulated by RNAstructure (Fig.1). This combination is compatible with the assembly method that XJTLU-CHINA promoted.
  
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This is a composite regulatory part that consisting of T7 promoter <partinfo>BBa_I13453</partinfo> and RNA Thermometer U6 <partinfo>BBa_K1824564</partinfo> for temperature induced gene expression at 37 degrees. The possible secondary structure of U6 was simulated by RNAstructure (Fig.1). This combination is compatible with the assembly method that XJTLU-CHINA promoted.
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[[Image:U6.jpg|360px]]
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<br>'''Figure 1:''' Possible secondary structure of RNAT U6.
  
 
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Latest revision as of 12:35, 6 September 2015

Consensus Promoter T7 + RNA Thermometer U6

This is a composite regulatory part that consisting of T7 promoter BBa_I13453 and RNA Thermometer U6 BBa_K1824564 for temperature induced gene expression at 37 degrees. The possible secondary structure of A1 was simulated by RNAstructure (Fig.1). This combination is compatible with the assembly method that XJTLU-CHINA promoted.

This is a composite regulatory part that consisting of T7 promoter BBa_I13453 and RNA Thermometer U6 BBa_K1824564 for temperature induced gene expression at 37 degrees. The possible secondary structure of U6 was simulated by RNAstructure (Fig.1). This combination is compatible with the assembly method that XJTLU-CHINA promoted.


U6.jpg
Figure 1: Possible secondary structure of RNAT U6.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 155
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal SpeI site found at 155
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
    Illegal BamHI site found at 141
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 155
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 155
  • 1000
    COMPATIBLE WITH RFC[1000]