Difference between revisions of "Part:BBa R0040:Design"

 
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<partinfo>BBa_R0040 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_R0040 SequenceAndFeatures</partinfo>
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===Design Notes===
 
===Design Notes===
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BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified      to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
 
BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified      to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
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===Source===
 
===Source===
  
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--What is the source of this part?--
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Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210.
  
 
===References===
 
===References===
  
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* Lutz R, Bujard H. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10.
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===Files===
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Revision as of 00:59, 5 June 2006


TetR repressible promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.



Source

Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.

References

  • Lutz R, Bujard H. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10.